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rabbit polyclonal antibodies against ampk α1  (Proteintech)


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    Structured Review

    Proteintech rabbit polyclonal antibodies against ampk α1
    3-BP induces <t>AMPK</t> phosphorylation and induces cell death. (A) The levels of CHOP, GRP78, AMPK-α and p-AMPK were detected in breast cancer MCF-7 and MDA-MB-231 cells treated with 3-BP (0, 40, 80 and 160 µmol/l) for 24 h via western blot analysis. (B) The expression levels of Bax in MCF-7 cells and caspase-3 protein in MDA-MB-231 cells were detected via western blotting in cells treated with 80 µmol/l 3-BP and 200 ng/ml TRAIL. 3-BP, 3-bomopyruvate.
    Rabbit Polyclonal Antibodies Against Ampk α1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against ampk α1/product/Proteintech
    Average 96 stars, based on 189 article reviews
    rabbit polyclonal antibodies against ampk α1 - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "3-Bromopyruvate sensitizes human breast cancer cells to TRAIL-induced apoptosis via the phosphorylated AMPK-mediated upregulation of DR5"

    Article Title: 3-Bromopyruvate sensitizes human breast cancer cells to TRAIL-induced apoptosis via the phosphorylated AMPK-mediated upregulation of DR5

    Journal: Oncology Reports

    doi: 10.3892/or.2018.6644

    3-BP induces AMPK phosphorylation and induces cell death. (A) The levels of CHOP, GRP78, AMPK-α and p-AMPK were detected in breast cancer MCF-7 and MDA-MB-231 cells treated with 3-BP (0, 40, 80 and 160 µmol/l) for 24 h via western blot analysis. (B) The expression levels of Bax in MCF-7 cells and caspase-3 protein in MDA-MB-231 cells were detected via western blotting in cells treated with 80 µmol/l 3-BP and 200 ng/ml TRAIL. 3-BP, 3-bomopyruvate.
    Figure Legend Snippet: 3-BP induces AMPK phosphorylation and induces cell death. (A) The levels of CHOP, GRP78, AMPK-α and p-AMPK were detected in breast cancer MCF-7 and MDA-MB-231 cells treated with 3-BP (0, 40, 80 and 160 µmol/l) for 24 h via western blot analysis. (B) The expression levels of Bax in MCF-7 cells and caspase-3 protein in MDA-MB-231 cells were detected via western blotting in cells treated with 80 µmol/l 3-BP and 200 ng/ml TRAIL. 3-BP, 3-bomopyruvate.

    Techniques Used: Phospho-proteomics, Western Blot, Expressing

    The AMPK inhibitor Compound C attenuates the effects of 3-BP on breast cancer cells. MCF-7 and MDA-MB-231 cells were treated with 1 µmol/l Compound C (Com C), 80 µmol/l 3-BP, and 200 ng/ml TRAIL, or both 3-BP and TRAIL, as indicated. (A) Cell viability was determined using an MTT assay. (B) Cell morphology was examined via light microscopy and apoptosis rate was determined using the PI staining method and flow cytometry. Data are expressed as the mean ± standard error of the mean (n=3). TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; 3-BP, 3-bomopyruvate.
    Figure Legend Snippet: The AMPK inhibitor Compound C attenuates the effects of 3-BP on breast cancer cells. MCF-7 and MDA-MB-231 cells were treated with 1 µmol/l Compound C (Com C), 80 µmol/l 3-BP, and 200 ng/ml TRAIL, or both 3-BP and TRAIL, as indicated. (A) Cell viability was determined using an MTT assay. (B) Cell morphology was examined via light microscopy and apoptosis rate was determined using the PI staining method and flow cytometry. Data are expressed as the mean ± standard error of the mean (n=3). TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; 3-BP, 3-bomopyruvate.

    Techniques Used: MTT Assay, Light Microscopy, Staining, Flow Cytometry

    AMPK induces ER stress and sensitizes breast cancer cells to TRAIL in response to treatment with 3-BP. (A) Cells treated with medium (Control), 1 µmol/l Compound C (Com C) or Compound C combined with 80 µmol/l 3-BP for 24 h were investigated via flow cytometry. (B) MCF-7 and MDA-MB-231 cells pre-treated with 1 µmol/l Compound C for 1 h were subsequently treated with 0, 40, 80 or 160 µmol/l 3-BP for 24 h. The expression levels of AMPK, GRP78, CHOP and DR5 were investigated with western blotting. (C) Cells pre-treated with or without 1 µmol/l Compound C for 1 h, were treated with medium, Compound C, 80 µmol/l 3-BP, 200 ng/ml TRAIL or both 3-BP and TRAIL, as indicated, for 24 h. The expression levels of Bax and Bcl-2 were determined in MCF-7 cells and caspase-3 was investigated in the MDA-MB-231 cells by western blotting. β-actin served as loading control. TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; 3-BP, 3-bomopyruvate; DR5, death receptor 5.
    Figure Legend Snippet: AMPK induces ER stress and sensitizes breast cancer cells to TRAIL in response to treatment with 3-BP. (A) Cells treated with medium (Control), 1 µmol/l Compound C (Com C) or Compound C combined with 80 µmol/l 3-BP for 24 h were investigated via flow cytometry. (B) MCF-7 and MDA-MB-231 cells pre-treated with 1 µmol/l Compound C for 1 h were subsequently treated with 0, 40, 80 or 160 µmol/l 3-BP for 24 h. The expression levels of AMPK, GRP78, CHOP and DR5 were investigated with western blotting. (C) Cells pre-treated with or without 1 µmol/l Compound C for 1 h, were treated with medium, Compound C, 80 µmol/l 3-BP, 200 ng/ml TRAIL or both 3-BP and TRAIL, as indicated, for 24 h. The expression levels of Bax and Bcl-2 were determined in MCF-7 cells and caspase-3 was investigated in the MDA-MB-231 cells by western blotting. β-actin served as loading control. TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; 3-BP, 3-bomopyruvate; DR5, death receptor 5.

    Techniques Used: Control, Flow Cytometry, Expressing, Western Blot



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    rabbit polyclonal antibodies against ampk α1  (Proteintech)
    96
    Proteintech rabbit polyclonal antibodies against ampk α1
    3-BP induces <t>AMPK</t> phosphorylation and induces cell death. (A) The levels of CHOP, GRP78, AMPK-α and p-AMPK were detected in breast cancer MCF-7 and MDA-MB-231 cells treated with 3-BP (0, 40, 80 and 160 µmol/l) for 24 h via western blot analysis. (B) The expression levels of Bax in MCF-7 cells and caspase-3 protein in MDA-MB-231 cells were detected via western blotting in cells treated with 80 µmol/l 3-BP and 200 ng/ml TRAIL. 3-BP, 3-bomopyruvate.
    Rabbit Polyclonal Antibodies Against Ampk α1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against ampk α1/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal antibodies against ampk α1 - by Bioz Stars, 2026-02
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    polyclonal rabbit antibody against the ampk-α1 and -α2 isoforms  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc polyclonal rabbit antibody against the ampk-α1 and -α2 isoforms
    3-BP induces <t>AMPK</t> phosphorylation and induces cell death. (A) The levels of CHOP, GRP78, AMPK-α and p-AMPK were detected in breast cancer MCF-7 and MDA-MB-231 cells treated with 3-BP (0, 40, 80 and 160 µmol/l) for 24 h via western blot analysis. (B) The expression levels of Bax in MCF-7 cells and caspase-3 protein in MDA-MB-231 cells were detected via western blotting in cells treated with 80 µmol/l 3-BP and 200 ng/ml TRAIL. 3-BP, 3-bomopyruvate.
    Polyclonal Rabbit Antibody Against The Ampk α1 And α2 Isoforms, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit antibody against the ampk-α1 and -α2 isoforms/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit antibody against the ampk-α1 and -α2 isoforms - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    3-BP induces AMPK phosphorylation and induces cell death. (A) The levels of CHOP, GRP78, AMPK-α and p-AMPK were detected in breast cancer MCF-7 and MDA-MB-231 cells treated with 3-BP (0, 40, 80 and 160 µmol/l) for 24 h via western blot analysis. (B) The expression levels of Bax in MCF-7 cells and caspase-3 protein in MDA-MB-231 cells were detected via western blotting in cells treated with 80 µmol/l 3-BP and 200 ng/ml TRAIL. 3-BP, 3-bomopyruvate.

    Journal: Oncology Reports

    Article Title: 3-Bromopyruvate sensitizes human breast cancer cells to TRAIL-induced apoptosis via the phosphorylated AMPK-mediated upregulation of DR5

    doi: 10.3892/or.2018.6644

    Figure Lengend Snippet: 3-BP induces AMPK phosphorylation and induces cell death. (A) The levels of CHOP, GRP78, AMPK-α and p-AMPK were detected in breast cancer MCF-7 and MDA-MB-231 cells treated with 3-BP (0, 40, 80 and 160 µmol/l) for 24 h via western blot analysis. (B) The expression levels of Bax in MCF-7 cells and caspase-3 protein in MDA-MB-231 cells were detected via western blotting in cells treated with 80 µmol/l 3-BP and 200 ng/ml TRAIL. 3-BP, 3-bomopyruvate.

    Article Snippet: The rabbit polyclonal antibodies against AMPK-α1 (1:500 dilution; cat. no. 10929-2-AP), Bax (1:5,000 dilution; cat. no. 50599-2-Ig) and Bcl-2 (1:1,000 dilution; cat. no. 12789-1-AP) were supplied by ProteinTech Group, Inc. (Chicago, IL, USA).

    Techniques: Phospho-proteomics, Western Blot, Expressing

    The AMPK inhibitor Compound C attenuates the effects of 3-BP on breast cancer cells. MCF-7 and MDA-MB-231 cells were treated with 1 µmol/l Compound C (Com C), 80 µmol/l 3-BP, and 200 ng/ml TRAIL, or both 3-BP and TRAIL, as indicated. (A) Cell viability was determined using an MTT assay. (B) Cell morphology was examined via light microscopy and apoptosis rate was determined using the PI staining method and flow cytometry. Data are expressed as the mean ± standard error of the mean (n=3). TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; 3-BP, 3-bomopyruvate.

    Journal: Oncology Reports

    Article Title: 3-Bromopyruvate sensitizes human breast cancer cells to TRAIL-induced apoptosis via the phosphorylated AMPK-mediated upregulation of DR5

    doi: 10.3892/or.2018.6644

    Figure Lengend Snippet: The AMPK inhibitor Compound C attenuates the effects of 3-BP on breast cancer cells. MCF-7 and MDA-MB-231 cells were treated with 1 µmol/l Compound C (Com C), 80 µmol/l 3-BP, and 200 ng/ml TRAIL, or both 3-BP and TRAIL, as indicated. (A) Cell viability was determined using an MTT assay. (B) Cell morphology was examined via light microscopy and apoptosis rate was determined using the PI staining method and flow cytometry. Data are expressed as the mean ± standard error of the mean (n=3). TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; 3-BP, 3-bomopyruvate.

    Article Snippet: The rabbit polyclonal antibodies against AMPK-α1 (1:500 dilution; cat. no. 10929-2-AP), Bax (1:5,000 dilution; cat. no. 50599-2-Ig) and Bcl-2 (1:1,000 dilution; cat. no. 12789-1-AP) were supplied by ProteinTech Group, Inc. (Chicago, IL, USA).

    Techniques: MTT Assay, Light Microscopy, Staining, Flow Cytometry

    AMPK induces ER stress and sensitizes breast cancer cells to TRAIL in response to treatment with 3-BP. (A) Cells treated with medium (Control), 1 µmol/l Compound C (Com C) or Compound C combined with 80 µmol/l 3-BP for 24 h were investigated via flow cytometry. (B) MCF-7 and MDA-MB-231 cells pre-treated with 1 µmol/l Compound C for 1 h were subsequently treated with 0, 40, 80 or 160 µmol/l 3-BP for 24 h. The expression levels of AMPK, GRP78, CHOP and DR5 were investigated with western blotting. (C) Cells pre-treated with or without 1 µmol/l Compound C for 1 h, were treated with medium, Compound C, 80 µmol/l 3-BP, 200 ng/ml TRAIL or both 3-BP and TRAIL, as indicated, for 24 h. The expression levels of Bax and Bcl-2 were determined in MCF-7 cells and caspase-3 was investigated in the MDA-MB-231 cells by western blotting. β-actin served as loading control. TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; 3-BP, 3-bomopyruvate; DR5, death receptor 5.

    Journal: Oncology Reports

    Article Title: 3-Bromopyruvate sensitizes human breast cancer cells to TRAIL-induced apoptosis via the phosphorylated AMPK-mediated upregulation of DR5

    doi: 10.3892/or.2018.6644

    Figure Lengend Snippet: AMPK induces ER stress and sensitizes breast cancer cells to TRAIL in response to treatment with 3-BP. (A) Cells treated with medium (Control), 1 µmol/l Compound C (Com C) or Compound C combined with 80 µmol/l 3-BP for 24 h were investigated via flow cytometry. (B) MCF-7 and MDA-MB-231 cells pre-treated with 1 µmol/l Compound C for 1 h were subsequently treated with 0, 40, 80 or 160 µmol/l 3-BP for 24 h. The expression levels of AMPK, GRP78, CHOP and DR5 were investigated with western blotting. (C) Cells pre-treated with or without 1 µmol/l Compound C for 1 h, were treated with medium, Compound C, 80 µmol/l 3-BP, 200 ng/ml TRAIL or both 3-BP and TRAIL, as indicated, for 24 h. The expression levels of Bax and Bcl-2 were determined in MCF-7 cells and caspase-3 was investigated in the MDA-MB-231 cells by western blotting. β-actin served as loading control. TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; 3-BP, 3-bomopyruvate; DR5, death receptor 5.

    Article Snippet: The rabbit polyclonal antibodies against AMPK-α1 (1:500 dilution; cat. no. 10929-2-AP), Bax (1:5,000 dilution; cat. no. 50599-2-Ig) and Bcl-2 (1:1,000 dilution; cat. no. 12789-1-AP) were supplied by ProteinTech Group, Inc. (Chicago, IL, USA).

    Techniques: Control, Flow Cytometry, Expressing, Western Blot